Our software improves the throughput by up to 8x of rtPCR tests by providing instructions on how to mix multiple patient samples and using combinatorics to quickly identify the infected patients in a large batch
Increase your current testing capacity by up to 8x with no new hardware or materials
Rather than testing patient samples independently, PCR Pooling provides lab technicians with a protocol detailing how to mix patient samples into pools. Testing the pools (instead of patients independently) makes it possible to quickly and reliably infer which patients are healthy and infected, using mathematical tools.
The conventional method of independent sample screening creates a bottleneck where samples are queued until a well (or slot) in a PCR machine becomes available. PCR Pooling widens this bottleneck through informed mixing of patient samples into pools.
There are a number of advantages to pooling samples, some of them are:
This protocol works best when two conditions are fulfilled:
False results are mitigated in software
Desired Sensitivity and specificity is configurable
Bayesian statistics are used to classify samples only once the desired accuracy is reached.
Replicates are dynamically run to reach desired specificity
Errors are tracked for each sample and replicated if necessary
A customizable number of positive and negative controls
Controls can be randomised, or in a dedicated well
Suppport for many robot file formats
Manual input supported with printable protocols
Support for standard rt-PCR file outputs
Manual override available at all steps
Samples can be added even after the experiment has started
The software will prioritise older samples to reduce latency